Title : Ergosterol, a mushroom sterol suppresses bisphenol A-induced BV2 microglial cell inflammation via NF-?B signaling pathway
Background: Bisphenol A (BPA) is widely used in the production of polycarbonate plastics and linings in food cans. BPA has been reported to have neurotoxic properties that may increase the risk of neurodegenerative diseases by inducing neuroinflammation. Auricularia polytricha (AP) is an edible mushroom with several medicinal properties. In particular, it is beneficial to the immune and nervous systems.
Methods: The anti-neuroinflammatory effects of AP extracts against BPA-induced inflammation of BV2 microglial cells were investigated. mRNA and protein levels of pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin 1-β, and IL-6 were measured by reverse transcription-quantitative real time PCR and enzyme-linked immunosorbent assay, respectively. The effect of AP extracts on NF-κB signaling was evaluated in BPA-induced BV2 cell activation. The neuroprotective effect of AP extracts via the modulation of microglial inflammation was determined by treating HT-22 hippocampal cells with BV2-conditioned media. Furthermore, ergosterol was isolated, and its biological activity was tested on BPA-induced BV2 cells.
Results: Hexane (APH) and ethanol (APE) extracts of AP inhibited BPA-induced neuroinflammation in BV2 microglial cells by reducing microglial activation and the expression of pro-inflammatory cytokines. These anti-inflammatory effects were regulated by the NF-κB signaling pathway. In addition, APH and APE exhibited antioxidative effects by increasing the activity of SOD-1 enzyme and restoring the accumulation of reactive oxygen species (ROS) in BPA-induced BV2 cells. Moreover, the conditioned medium prepared using BPA-induced BV2 cells demonstrated that the presence of APH or APE could attenuate ROS production in HT-22 hippocampal cells. Further, ergosterol showed anti-inflammatory and antioxidative effects via NF-κB signaling and upregulation of SOD-1 activity, respectively.
Conclusion: AP extracts and ergosterol attenuated neuroinflammation against BPA induction in BV2 microglial cells through the NF-κB signaling pathway. Moreover, these natural compounds serve as neuroprotective agents by modulating microglial activation.
What will audience learn from your presentation?
- This study demonstrates the toxicity of bisphenol A in microglial cell activation, which is one of the most common causes of neurodegenerative disorders.
- This finding exhibits a novel therapeutic effect of mushroom extracts and bioactive compound for neuroinflammatory inhibition
- This research will provide preliminary data for anti-neuroinflammatory drug development from natural products.