HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of International Conference on Neurology and Brain Disorders

October 24 -26, 2022

October 24 -26, 2022 | Orlando, Florida, USA
INBC 2022

Abdul Wahab

Speaker at Neurology and Brain Disorders 2022 - Abdul Wahab
Department of Microbiology, Pakistan
Title : A novel metabarcoded 18S ribosomal DNA sequencing tool for the detection of Plasmodium species in malaria positive patients

Abstract:

Malaria elimination strategies demand constant monitoring of the parasite population for genetic variations that necessitate a public health response, such as a new form of drug resistance. Malaria control relies mainly on rapid and precise diagnosis, followed by successful treatment. Malaria diagnosis must be made as soon as possible to provide optimal disease management and surveillance. In every situation, the accuracy of malaria diagnosis is essential, as misdiagnosis can result in severe morbidity and mortality. Here, we describe a novel, high through put method using an Illumina Mi-Seq platform to demonstrate the proportions of Plasmodium species in metabarcoded DNA samples derived from human malaria patients. We reported a unique, high throughput approach for determining the ratio of Plasmodium species in metabarcoding DNA samples generated from human malaria patients using an Illumina Mi-Seq. Positive control gDNA from P. falciparum and P. vivax was used to mock DNA pols of parasites to test the assay direction threshold for each species. The several mock pools indicate the accuracy of the detection abilities and the proportion of each species present. The technique was subsequently used on malaria-positive patient samples to determine the species composition of Plasmodium populations in Pakistan's Punjab region and the tribal territories of the Auragzai Agency Border (ShanaweriZargiri) Ali Masjid Landi-Kotal Khyber Agency border the Pakistan-Afghanistan. The deep amplicon sequencing approach contrasts with an immunochromatographic test, commonly utilized for diagnosis in the region. According to deep amplicon sequencing, P. vivax was present in 69.8 percent of the patients, P. falciparum in 29.5 percent, and mixed infection in 0.7 percent of the cases. Plasmodium vivax was found in 65.6 percent of patients, P. falciparum in 27.4 percent, mixed infection in 0.7 percent of patients, and 6.32 percent of positive malaria cases were negative in immunochromatographic but positive in deep amplicon sequencing.

Overall, metabarcoding DNA sequencing improves diagnosis accuracy, resulting in a significant increase in Plasmodium infection prevalence estimates. The use of metabarcoding DNA in next-generation sequencing could help in Plasmodium infection diagnosis, surveillance, treatment, and control, as well as research into parasite biology.

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