Title : Neuroprotective effect of L-Theanine against Tramadol Induced Parkinson’s like symptoms in experimental Rats
Abstract:
Introduction
Parkinson’s disease (PD) is a chronic neurodegenerative disorder triggered by degeneration of dopaminergic neurons in substantia nigra pars compacta (SNpc). Low antioxidant level, mitochondrial failure and neuroinflammation are the major pathological mechanisms.
Material and Methods:
Experimental Animals: Wistar Rats (180-200 g).
Drugs and Chemicals: Tramadol, L- theanine, Naloxone and Sinemet.
Experimental procedure: The animals were divided into 7 groups. Group 1 served as Normal control.
Group 2 received Tramadol (50 mg/kg, i.p.) daily for 28 days. Group 3, 4 and 5 received L-theanine (25, 50 and 100 mg/kg; p.o.) from 14 to 28 day prior to the tramadol administration. Group 6 received Sinemet [Levodopa and Carbidopa] (36mg/kg; p.o.) from day14today28priortothetramadoladministration. Group 7 received Naloxone (0.4mg/kg; i.p.) from day 14 to day 28 prior to the tramadol administration.Behavioral observations were done on 1, 7, 14, 21 and 28 day after tramadol treatment. On 29 day, animals were sacrificed and striatum was isolated for biochemical, neuroinflammation, histopathological and neurotransmitters analysis.
Results:
Administration of tramadol (50 mg/ kg, i.p.) for 28 days in rats produces impaired motor functions and locomotor activity as evidenced by rotarod, open field, narrow beam walks and grip strength performance. In addition, there was increased oxidative stress (MDA, nitrite) and neuroinflammatory markers (TNF-α, IL-1β and IL-17) and decreased levels of catecholamines, GABA and glutamate. The treatment drug L-theanine at dose (25, 50, 100 mg/kg) significantly and dose- dependently improved alterations in motor impairments and locomotor activity, attenuated oxidative stress, neuroinflammatory markers and restored catecholamines, GABA and glutamate level in striatum.
Conclusion:
Chronic tramadol administration produces impaired motor functions, increased oxidative stress, neuroinflammation and altered neurotransmitters level was significantly ameliorated by L-theanine, through antioxidant, anti-inflammatory and neuroprotective mechanisms.
What will audience learn from your presentation?
Audience will be able to know the effects of tramadol in central nervous system followed by treatment of L-theanine
