HYBRID EVENT: You can participate in person at Baltimore, Maryland, USA or Virtually from your home or work.

10th Edition of International Conference on Neurology and Brain Disorders

October 21-23, 2024

October 21 -23, 2024 | Baltimore, Maryland, USA
INBC 2018

Kruchinina Margarita Vitalievna

Speaker at Brain Disorders Conference - Kruchinina Margarita Vitalievna
Research Institute of internal and Preventive Medicine- Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Russian Federation
Title : The role of red blood cell as a participant in vasoregulation in the patients with ischemic stroke


The aim of the work was to assess the red blood cell (RBC) role as a participant in vasoregulation in the patients with ischemic stroke (IS) from two points: first, as a direct deliverer of NO and a stimulator of NO-release via RBC generated ATP.

Materials and methods. 467 patients (49,1+4,7 years old) with IS at its sub-acute or residual stage and 35 healthy controls were included into the study. The study of RBC properties was performed by dielectrophoresis, chromatography, 31P NMR spectroscopy. Changes in the content of Hb complexes were studied by Raman spectroscopy.

Results. IS patients had marked disturbances of RBC deformability (low amplitude of deformation at the background of  high summarized rigidity, viscosity), RBC’s membrane characteristics (high electrical conductivity, low capacitance), low surface electric charge (reflected as the low dipole moment, the speed of RBC movement to electrodes) (p<0,0001-0,05), the last one correlated with the level of Von Wilebrand factor (r=0,72, p<0,033). These changes were associated with high levels of cholesterol fraction, an index of cholesterol/phospholipids in RBC membranes against decrease in total lipids, easily oxidable PHL, omega-3 index (p<0,0001-0,02).

We found low levels of intracellular macroergs (2,3-DPG, alpha-, beta-, gamma-ATP) in rigid RBC in IS patients than those in controls (p<0,0001-0,05). In patients with IS the content of the Hb-NO (II) complexes were sharply reduced (p<0,01-0,05), which led to a significant reduction in oxygen discharge and reflected the decline reserves of erythrocyte NO. Under hypoxic conditions as in the case of ischemic stroke, it is apparent that the RBC does release both ATP and NO. Is it possible that one of the molecules (ATP) is designed to stimulate NO in the endothelium, while the NO derived directly from the RBC has another function - to inhibit platelet aggregation in the presence of high levels of ATP being secreted by the RBC under hypoxic conditions. At the same time we revealed elevated levels of platelet aggregation with different types of inductors in patients with IS (p<0,01-0,02) as well as leukocytic-and-platelet aggregation (p=0,04). Probably the changed parameters of RBC in patients with IS contribute to the altered vasoregulation.

Conclusion. The decline reserves of intracellular NO, macroergs in rigid RBC in patients with ischemic stroke has been revealed. This fact as well as altered release of these compounds, associated with low deformability of RBC, may lead to modified  vasoregulation in ischemic stroke.


Kruchinina Margarita Vitalievna ,  graduated from Novosibirsk Medical Institute, Russia, "medical case" in 1989, specialty "doctor", diploma with honors.

I studied in Residency in Therapy in 1989-1991, Postgraduate course on Therapy in 1991-1994, Doctorate in 2002-2005 (Institute of Internal Medicine SB RAMS, Novosibirsk).

I defended my doctoral thesis in 2007(Doctor of Medical Sciences, Diploma DDN № 003697).

From 2009 to the present time I am Leading researcher of  Research Institutе of Internal and Preventive Medicine – Branch of Federal State Budget Scientefic Institution The Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

I am the author of more than 120 publications, including 1 monograph, 5 teaching aids, 8 patents.

Areas of scientific interests are microcirculation, rheology, membrane disorders in cerebrovascular, cardiovascular pathology, diagnosis of diffuse liver pathology, oncopathology.