Title : Properties of the ovocystatin in APP/PS1 mice model
Abstract:
Cysteine proteases inhibitors play an important role in the neurodegenerative disorders. Cystatin isolated from chicken egg white, called ovocystatin has been widely used in pharmaceutical and medical research due to its structural and biological similarities to human cystatin C. Moreover, it has been recently suggested that ovocystatin has procognitive properties in young rats (Sta?czykiewicz et al, 2017a), as well as may prevent aging-related cognitive impairment (Sta?czykiewicz et al., 2017b). Thus, the presented study focused on the histochemical and biological evaluation of the ovocystatin properties in APP/PS1 transgenic mice, including cognitive functions’ assessment. The study was conducted on transgenic B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax mice. Ovocystatin was administered intraperitoneally for four weeks (at a dose of 40/ mouse) to 35-weeks-old transgenic (AD) and wild type (NCAR) mice. The locomotor activity and cognitive functions were determined using an actimeter and the Morris water maze test, respectively. Histopathological evaluation comprised antibodies directed against Beta-Amyloid (1:400, SIG-39320-1000, Covance), Tau (1:4000, AHB0042, Invitrogen), RBFOX3/NeuN (1:1000, NBP1-77686, Novus Biologicals) and CD107b (1:100, 108502, BioLegend). The study was conducted within the framework of the POIG 01.03.01-00-133/08 „Innovative Technologies of production of biopreparations based on new generations of eggs (OVOCURA)”- Task 8. Assessing the biological activity of the obtained biopreparations. It was expected that the study may provide a significant contribution to the development of research on neurodegenerative disorders, by describing their effects on cognitive function and biological activity. The authors will present the results of the study and key findings.
