Title : Intraperitoneal injection of VUF-8430 in mice induced a decrease in CREB and CREB phosphorylation levels in the cerebellar vermis and prefrontal cortex, but not in amygdala and hippocampus.
Histamine H4 receptors agonist VUF-8430 have been shown participate in emotional memory. In the present study, the aim was to verify if the intraperitoneal (i.p.) injection of VUF-8430 (1.48 nmol; 10 ml / kg) in mice, acts on the protein synthesis required in memory consolidation processes, activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala and hippocampus) and the cerebellar vermis, a structure that is recently also had its role attributed to emotional memory, using the Western blotting technique. The results obtained demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex of the animals, suggesting that this dose impaired the activation of cell signaling pathways in these structures. However, there was no change in this protein expression in the amygdala and hippocampus. These results can be attributed to a feedback between anatomically distinct neural circuits that promote the modulation of signaling pathways associate to emotional memory.