4th International Conference on Neurology and Brain Disorders

September 09-11, 2021

September 09 -11, 2021 | Virtual Event
INBC 2017

Niall Finnerty

Speaker at International Conference on Neurology and Brain Disorders 2017 - Niall Finnerty
Maynooth University, Ireland
Title : Continuous real-time monitoring of brain extracellular fluid using microamperometric sensors and their application in a humanized mouse model of Parkinson’s disease

Abstract:

In vivo amperometry is an electroanalytical technique whereby a sensitive and selective microelectrochemical sensor is implanted into a particular brain region for continuous real-time recordings of a particular neurochemical. A suitable potential is applied to the sensor surface, generating a faradaic current that is proportional to the concentration of the detected neurochemical. This presentation will discuss the in vitro development and characterization of an electrochemical sensor through to its subsequent in vivo characterization in the rodent brain and its eventual application in an animal model of disease. Current work is focusing on the ability of this technique to measure neurochemical transitions in a humanized mouse model of Parkinson’s disease (PD). In summary, PD patient-derived induced pluripotent stem cells (iPSC) are differentiated into dopaminergic neurons and transplanted into the striatum of NOD SCID mice to facilitate anatomical integration over a couple of months. To date, there has been limited translation from existing animal models of PD to clinical neuroprotection in human populations. A large number of potentially neuroprotective compounds from a broad range of pharmacological groups have been identified in rodent and primate models, however, none have proven neuroprotective during clinical testing. The general consensus is that this disparity is mainly due to the aetiopathogenic diversity of PD and humanised models can potentially bridge the gap between standard pre-clinical animal models of PD and clinical translation. This humanised mouse model will facilitate unprecedented access to perform amperometric recordings within the microenvironment of transplanted PD human cells.

Audience take away:

• How to develop and characterize a sensitive and selective amperometric sensor in vivo.

• How to characterize an amperometric sensor in rodent brain extracellular fluid. 

• The application of amperometry to measure from within transplanted PD human cells.

• Knowledge of work being performed by SysMedPD consortium on the development of a neuroprotective treatment to slow down the progression of PD.

Biography:

Niall Finnerty is a Research Fellow at Maynooth University in Ireland who has over 13 years’ experience in the development and characterisation of microelectrochemical sensors for neurochemical analysis. His research portfolio to date includes in vitro and in vivo investigations using microsensors for the real time measurement of nitric oxide, oxygen, hydrogen peroxide, superoxide and pH with applications in animal models of Parkinson’s disease and schizophrenia. Furthermore, he spent four years investigating the translation of a tissue oxygen sensor from an early stage research tool into a commercial product suitable for clinical tissue monitoring. Currently, he is a principal investigator within the Horizon 2020 funded SysMedPD consortium, focusing on the continuous real time monitoring of reactive oxygen and nitrogen species in a humanized mouse model of Parkinson’s disease.

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