HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of International Conference on Neurology and Brain Disorders

October 24 -26, 2022

October 24 -26, 2022 | Orlando, Florida, USA
INBC 2022

Rizwan Khan

Speaker at Neurology and Brain Disorders 2022 - Rizwan Khan
Western Sydney University, Australia
Title : Immunogenetic Detection of Alzheimer’s Disease

Abstract:

Alzheimer’s disease (AD) is on a rapid rise in the aged population with typical hallmarks of extracellular Aβ deposits and intracellular neurofibrillary tangles (NFT) composed of hyperphosphorylated tau proteins. Positron emission tomography imaging and screening for Aβ peptides and hyperphosphorylated tau in the cerebrospinal fluid are currently considered the ‘gold standard’ to confirm AD. However, the current diagnostic modalities have great limitations as these are highly expensive, difficult to apply in health system set up and often lack reproducibility. Immune/inflammatory responses play a key role in maintaining brain homeostasis, however, disturbances associated with inflammatory responses confirm the role of mediators of inflammatory/immune response in neurodegeneration. The major aim of this study was to investigate abnormalities associated with the immune responses in AD patients and in a mouse model of AD (APP/PS1) and to establish a molecular “fingerprint” specific for early detection of AD. Initially, using next generation sequencing, we investigated the B-cell receptors (BCR) in APP/PS1 and compared with wild type littermates. We observed a higher mutation rate associated with the APP/PS1BCR repertoire. In addition, physicochemical analysis showed that the APP/PS1 BCR repertoire displayed higher instability indices of the clones, suggesting a defect in the immune responses against antigens. Of importance, most of the somatic hypermutation homed to CDR1, CDR2 as well as FR2 and FR3Finally, our mass spectrometry analysis indicated that most of the identified proteins were associated with the immune system and were significantly down regulated. Taken together, these results confirm a generalized faulty immune system in AD and more specifically, highlight a defective isotype switch and affinity maturation responsible for the molecular immunological diversity. Our novel findings and strategies will help us pave the way to better understand the crucial role of Immune system in AD and develop a robust early diagnostic test for AD.

What will audience learn from your presentation?

  • Our current study is focused on developing a diagnostic test for AD that has been long awaited.
  • Our current study helps provide insights into somatic hypermutation and isotype switch associated with AD,
  • Such a study has never been published till date and opens new horizons in the diagnosis of AD

 

 

 

Biography:

Mr. Rizwan Khan comes from a Life Science background and is currently a 3rd year Ph.D. student at the School of Medicine, Western Sydney University. He completed his bachelor’s degree with a major in Biotechnology and a Master’s degree in Biochemistry from Osmania University, India. Rizwan’s research focused on the biochemical and textural characteristics of both sweet corn and wheat. His current research focuses on developing an early diagnostic test for Alzheimer’s disease. Other research interests of his include protein misfolding, neurodegenerative disorders and immunology. Rizwan has published 2 papers published in the WJPLS and 1 paper in Frontiers in Veterinary Science.

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